Transport of glucose in isolated goat ileum preparation: an alternative research tool for absorption study.

Isolated preparat ion are commonly used to study the effect of drugs on specific type of recep tor, fo r b ioassay of d rug , fo r characterization of specific type of receptor or i ts subtypes , for pharmacokinet ic s tudy, to de te rmine concent ra t ion response curve of agonist, to study the antagonism of drug and in new drug discovery (1). The Committee for the purpose of control and supervis ion of exper imenta l an imals (CPCSEA) has ins t ruc ted un ivers i t i es and ins t i tu t ions to reduce the use o f l abora tory an imals fo r teach ing and exper imenta l purpose . There are ex tens ive ly use o f the l abora tory animals eg. rat , mice, guinea pig, frogs for per forming the absorp t ion / t ranspor t s tudy of d rug (2 , 3 ) . There i s a requ i rement o f permiss ion f rom CPCSEA for per forming these experiments on laboratory animals. So th i s has d r iven us to conduc t a l t e rna t ive isola ted t i ssue (goat i leum) exper iment .